Microfluidic production of EV-like liposomes and characterization for studying metastasis and cell-to-cell communication

Extracellular vesicles (EVs) are cellular messengers that transport material to recipient cells.
In recent years, the role of cancer cell-derived EVs in driving metastatic progression has been reported. Better understanding of this process could help to disrupt EV-mediated pro-tumorigenic effects and/or exploit EVs as drug delivery systems.
The controlled production of artificial EV-like liposomes represents a new avenue that might help to understand how cancer cells communicate and metastasize.

The purpose of this research is to develop optimized synthetic EV-like liposomes through microfluidics using the nanoprecipitation method. These vesicles are going to be used to  mimic and model cancer cell-derived EVs in a metastatic context and serve as vehicles for drug delivery.

The role of the master degree student is to design and fabricate different microfluidic structures to synthetize and characterize liposomes to mimic EVs. The use of liposomes as EV models will be used to study cellular processes. These lipid nanoparticles or the liposomes’ surface will also be modified to integrate peptides, antibodies, proteins and genetics materials for drug delivery systems.

 

Connaissances requises

Applicants should have a strong background in science and engineering. In addition, it is desirable to have experience in the field of microfluidics, fluid dynamics and microfabrication. Experience in molecular biology is not required, but beneficial. 
 

Programme d'études visé

Maîtrise avec mémoire

Domaines de recherche

Santé

Financement

Research support:  19,000$ per year - Duration of research: 2 years

Autres informations

Starting : Automne 2020

Partners involved : McGill University Health Centre (MUHC).